Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
نویسندگان
چکیده
PCR amplification and sequencing of marker genes are a low-cost technique for monitoring prokaryotic eukaryotic microbial communities across space time but will work optimally only if environmental organisms match primer sequences exactly. In this study, we evaluated how well primers globally distributed short-read oceanic metagenomes.
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ژورنال
عنوان ژورنال: MSystems
سال: 2021
ISSN: ['2379-5077']
DOI: https://doi.org/10.1128/msystems.00565-21